Comments on: TWiV 66: Reverse transcription http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/ A netcast about viruses - the kind that make you sick Wed, 08 Feb 2012 21:43:00 +0000 hourly 1 http://wordpress.org/?v=3.3.1 By: Unexpected endogenous viruses http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-4406 Unexpected endogenous viruses Fri, 10 Dec 2010 13:01:18 +0000 http://www.twiv.tv/?p=957#comment-4406 [...] replication of retroviruses, a double-stranded DNA copy of the viral RNA genome is synthesized by reverse transcription and integrated into the genomes of the infected cell. When retroviral DNA is integrated into the [...] [...] replication of retroviruses, a double-stranded DNA copy of the viral RNA genome is synthesized by reverse transcription and integrated into the genomes of the infected cell. When retroviral DNA is integrated into the [...]

]]> By: zelo http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2625 zelo Sat, 23 Jan 2010 20:33:09 +0000 http://www.twiv.tv/?p=957#comment-2625 In the episode 66 some listener asked about the research made on a possible therapy to excise HIV form the cell genome. As far as I know so far there has been a successful experiment in which an evolved Cre recombinase (Tre) was developed to recognize the HIV-1 LTRs and excise the DNA sequence in between. The evolved Tre recombinase was tested in human cultured cells (HeLa cells) which had been transfected with a reporter gene (luciferase) within a sequence flanked by the HIV-1 LTRs. Still, as you mentioned in your reply one of the difficulties to overcome would be to selectively target those cells with the HIV provirus in their genome as in a number of cells the virus can remain latent for years. The other serious obstacle is how to introduce in the body enough enzyme or expression vector to successfully reach most infected cells while keeping under control the negative side effects caused by such invasive therapy.<br>For those interested in the experiments carried out to develop and test the Tre recombinase, this is the reference of the article in which the results were published:<br>Sarkar et al. HIV-1 proviral DNA excision using an evolved recombinase. Science (2007) vol. 316 (5833) pp. 1912<br>In that issue a short introduction commenting the experiments was also published: <br>Engelman. AIDS/HIV: A Reversal of Fortune in HIV-1 Integration. Science (2007) vol. 316 (5833) pp. 1855 In the episode 66 some listener asked about the research made on a possible therapy to excise HIV form the cell genome. As far as I know so far there has been a successful experiment in which an evolved Cre recombinase (Tre) was developed to recognize the HIV-1 LTRs and excise the DNA sequence in between. The evolved Tre recombinase was tested in human cultured cells (HeLa cells) which had been transfected with a reporter gene (luciferase) within a sequence flanked by the HIV-1 LTRs. Still, as you mentioned in your reply one of the difficulties to overcome would be to selectively target those cells with the HIV provirus in their genome as in a number of cells the virus can remain latent for years. The other serious obstacle is how to introduce in the body enough enzyme or expression vector to successfully reach most infected cells while keeping under control the negative side effects caused by such invasive therapy.
For those interested in the experiments carried out to develop and test the Tre recombinase, this is the reference of the article in which the results were published:
Sarkar et al. HIV-1 proviral DNA excision using an evolved recombinase. Science (2007) vol. 316 (5833) pp. 1912
In that issue a short introduction commenting the experiments was also published:
Engelman. AIDS/HIV: A Reversal of Fortune in HIV-1 Integration. Science (2007) vol. 316 (5833) pp. 1855

]]> By: dakezhang http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2622 dakezhang Sat, 23 Jan 2010 07:11:43 +0000 http://www.twiv.tv/?p=957#comment-2622 Got it, thx a lot! Got it, thx a lot!

]]> By: profvrr http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2620 profvrr Fri, 22 Jan 2010 19:45:16 +0000 http://www.twiv.tv/?p=957#comment-2620 I don't have a problem downloading this episode in iTunes, and it's<br>already been downloaded thousands of times. I'll email you a link to<br>the file if you are still having problems. I don't have a problem downloading this episode in iTunes, and it's
already been downloaded thousands of times. I'll email you a link to
the file if you are still having problems.

]]> By: dakezhang http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2619 dakezhang Fri, 22 Jan 2010 17:27:04 +0000 http://www.twiv.tv/?p=957#comment-2619 can not download your great podcast from itunes...wonder if there is something wrong....<br>thx... can not download your great podcast from itunes…wonder if there is something wrong….
thx…

]]> By: uberVU - social comments http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2618 uberVU - social comments Wed, 20 Jan 2010 19:01:45 +0000 http://www.twiv.tv/?p=957#comment-2618 <strong>Social comments and analytics for this post...</strong> This post was mentioned on Twitter by profvrr: This Week in Virology (TWiV) episode 66 is up: Reverse transcription http://bit.ly/4vHEcz... Social comments and analytics for this post…

This post was mentioned on Twitter by profvrr: This Week in Virology (TWiV) episode 66 is up: Reverse transcription http://bit.ly/4vHEcz...

]]> By: Tweets that mention TWiV 66: Reverse transcription -- Topsy.com http://www.twiv.tv/2010/01/17/twiv-66-reverse-transcription/comment-page-1/#comment-2611 Tweets that mention TWiV 66: Reverse transcription -- Topsy.com Mon, 18 Jan 2010 01:21:43 +0000 http://www.twiv.tv/?p=957#comment-2611 [...] This post was mentioned on Twitter by Vincent Racaniello, FluTrackers.com. FluTrackers.com said: RT @profvrr: This Week in Virology (TWiV) episode 66 is up: Reverse transcription http://bit.ly/4vHEcz [...] [...] This post was mentioned on Twitter by Vincent Racaniello, FluTrackers.com. FluTrackers.com said: RT @profvrr: This Week in Virology (TWiV) episode 66 is up: Reverse transcription http://bit.ly/4vHEcz [...]

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