TWiV 297: Ebola! Don’t panic

August 10, 2014

Reston virusHosts: Vincent RacanielloAlan DoveRich Condit, and Kathy Spindler

The TWiVites present an all-ebolavirus episode, tackling virology, epidemiology, and approaches to prevention and cure that are in the pipeline.

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Weekly Science Picks

Alan – Historical perspective on Ebola (Tara C Smith)
Rich – Tell the negative committee to shut up (Fanuel Muindi)
Kathy – Art of Night (Vimeo)
Vincent –
Is Ebola virus going to kill me? (John Skylar)

Listener Pick of the Week

Peter – Dr. Michael Saag interview by Dr. Virginia Campbell
David – PI Predictor (app, article, publication)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

  • poxdoc

    Reflecting on this episode, I regret not having included more about the function of individual Ebolavirus gene products in the introduction to these viruses, so this comment is an attempt to correct that omission. Follow along using the diagram of Ebolavirus on ViralZone: http://viralzone.expasy.org/viralzone/all_by_species/207.html

    The G protein is the major external viral structural glycoprotein. (Kathy nicely describes glycoproteins later in the episode.) The filamentous virus particle that everyone is now probably familiar with (see episode image) is surrounded by a lipid membrane that is derived by virus budding through the cell membrane during virus assembly, and the G protein is anchored to and protrudes from the viral membrane thus coating the outer surface of the virus. G protein is responsible for attachment and uptake of the virus into cells so it is critical for the initial stages of infection. It is also the major protein that the immune system “sees” on the virus particle during infection. Antibodies neutralize the virus by binding to this protein, and the different serotypes of virus have subtle differences in the structure of this protein so that most antibodies that react with the G protein from one species of Ebolavirus do not react well with another species of Ebolavirus. It is for these reasons that so much attention is focused on the G protein, in particular in the construction of vaccines and therapeutics.

    The nucleoprotein coats the RNA genome that resides within the virion membrane. I think it is reasonable to think of the RNA-nucleoprotein particle as a sort of virus chromosome within the particle. The nucleoprotein protects the RNA and also participates in transcription of the RNA into either mRNA or genomic RNA.

    The matrix protein lies between the RNA-nucleoprotein particle and the membrane and contacts both; it is a key structural component of the virus.

    The polymerase (called “L” for “large”) is responsible for copying the RNA genome into either mRNA, anti-genomic RNA, or ultimately more genomic RNA (see TWiV 60, “Making Viral RNA” (http://www.twiv.tv/2009/11/29/twiv-60-making-viral-rna/). It actually has two subunits; the other is VP35. Because cells don’t normally copy RNA into RNA, the viral polymerase is different than cellular polymerases and therefore potentially makes a good “druggable” target, as discussed later in the episode.

  • JohnSkylar

    Thanks for sharing my article, TWiV docs! I really like the rest of the episode, too–I’m sharing it with my own fanbase.

  • Trudy Bentley Rech

    I have shared this episode with friends and family in the hopes they will listen and any unwarranted fears will be assuaged. It was a long TWiV, however, it was one of the most interesting TWiVs ever. I worked in a county public health department in the communicable disease clinic and in epidemiology as a nurse. One of the key functions in public health is to do outreach and provide conduits to help inform our community about diseases, related risks and preventive measures. You provide ammunition for some of us who (although retired) continue to refer the public to the best resources. Thank you all for your expert efforts.

  • Laila Gwinyai

    Thanks for sharing your timely thoughts on this issue! I’m curious, though: why the dismissal of Richard Preston’s “The Hot Zone”? Does every science writer have to be a scientist? Isn’t it enough to have qualified sources and fact-checking? Yes, the book was luridly written and portrayed certain personalities in a less-than-flattering light, but I couldn’t find much mendacity in what he presented. If you could share any mistakes that stood out for you, it’d be appreciated, as this book still pulls a lot of social weight; not only because it was a best-seller 20 years ago — it’s required reading for countless secondary school curricula.

  • SSL

    Dr. Racaniello, can you please post a link for the scientist for science organization? Thank you!

  • SSL

    Never mind! I just missed it above.

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